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PCR targeting the 16S rRNA-encoding gene and chaperonin-60 (cpn60) showed that the plants were infected with phytoplasma subgroup16SrXIII-(A/I)I (SbGP/MPV). In the presented case, the multiplexed experiments allowed for early discovery of unspecific interactions and subsequent minimization of these, resulting in a protocol that provided improved enrichment of phosphopeptides. This research is waivered from trial registration because it was a retrospective analysis of medical records. Our objective is to review the available literature and analyse the patency rates and complications of various types of lower-extremity arteriovenous access.

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